[HTML][HTML] Apolipoprotein E containing high density lipoprotein stimulates endothelial production of heparan sulfate rich in biologically active heparin-like domains: a …
L Paka, Y Kako, JC Obunike, S Pillarisetti - Journal of Biological Chemistry, 1999 - ASBMB
Reduced heparin and heparan sulfate (HS) proteoglycans (PG) have been observed in both
inflammation and atherosclerosis. Methods to increase endogenous heparin and heparan
sulfate are not known. We found that incubation of endothelial cells with 500–1,000 μg/ml
high density lipoprotein (HDL) increased 35 SO 4 incorporation into PG by 1.5–2.5-fold. A
major portion of this increase was in HS and was the result of increased synthesis. Total PG
core proteins were not altered by HDL; however, the ratio of 35 SO 4 to [3 H] glucosamine …
inflammation and atherosclerosis. Methods to increase endogenous heparin and heparan
sulfate are not known. We found that incubation of endothelial cells with 500–1,000 μg/ml
high density lipoprotein (HDL) increased 35 SO 4 incorporation into PG by 1.5–2.5-fold. A
major portion of this increase was in HS and was the result of increased synthesis. Total PG
core proteins were not altered by HDL; however, the ratio of 35 SO 4 to [3 H] glucosamine …