Lipoprotein binding to canine hepatic membranes. Metabolically distinct apo-E and apo-B, E receptors.
Hepatic membranes from adult dog livers have receptors which bind to lipoproteins
containing the E apoprotein (the apo-E HDLc) but lack specific receptors for the apo-B-
containing low density lipoproteins (LDL). Scatchard analysis of direct binding data for 125I-
apo-E-HDLc revealed nonlinearity of the binding which could be resolved into two
components, suggesting the presence of two separate binding sites. The binding site for apo-
E HDLc that possessed the highest affinity (Kd= 0.23 x 10 (-9) M) was calcium-dependent …
containing the E apoprotein (the apo-E HDLc) but lack specific receptors for the apo-B-
containing low density lipoproteins (LDL). Scatchard analysis of direct binding data for 125I-
apo-E-HDLc revealed nonlinearity of the binding which could be resolved into two
components, suggesting the presence of two separate binding sites. The binding site for apo-
E HDLc that possessed the highest affinity (Kd= 0.23 x 10 (-9) M) was calcium-dependent …