[HTML][HTML] Isoform-specific purification and substrate specificity of the 5′-AMP-activated protein kinase
BJ Michell, D Stapleton, KI Mitchelhill… - Journal of Biological …, 1996 - ASBMB
The 5′-AMP-activated protein kinase (AMPK) mediates several cellular responses to
metabolic stress. Rat liver contains at least two isoforms of this enzyme, either α 1 or α 2
catalytic subunits together with β and γ noncatalytic subunits in a trimeric complex. The α 1
isoform is purified using a peptide substrate affinity chromatography column with ADR1 (222-
234) P229 (LKKLTRRPSFSAQ), corresponding to the cAMP-dependent protein kinase
phosphorylation site in the yeast transcriptional activator of the ADH2 gene, ADR1. This …
metabolic stress. Rat liver contains at least two isoforms of this enzyme, either α 1 or α 2
catalytic subunits together with β and γ noncatalytic subunits in a trimeric complex. The α 1
isoform is purified using a peptide substrate affinity chromatography column with ADR1 (222-
234) P229 (LKKLTRRPSFSAQ), corresponding to the cAMP-dependent protein kinase
phosphorylation site in the yeast transcriptional activator of the ADH2 gene, ADR1. This …