Incorporation of adeno-associated virus in a calcium phosphate coprecipitate improves gene transfer to airway epithelia in vitro and in vivo
RW Walters, D Duan, JF Engelhardt… - Journal of virology, 2000 - Am Soc Microbiol
RW Walters, D Duan, JF Engelhardt, MJ Welsh
Journal of virology, 2000•Am Soc MicrobiolAdeno-associated virus (AAV) is inefficient at infecting differentiated airway epithelia
because of a lack of receptors at the apical surface. We hypothesized that incorporation of
AAV in a calcium phosphate coprecipitate would circumvent this barrier. Interestingly,
coprecipitation of AAV type 2 improved gene transfer to differentiated human airway
epithelia in vitro and to the mouse lung in vivo. These results suggest that delivery of AAV as
a CaPicoprecipitate may significantly enhance its utility for gene transfer to the airway …
because of a lack of receptors at the apical surface. We hypothesized that incorporation of
AAV in a calcium phosphate coprecipitate would circumvent this barrier. Interestingly,
coprecipitation of AAV type 2 improved gene transfer to differentiated human airway
epithelia in vitro and to the mouse lung in vivo. These results suggest that delivery of AAV as
a CaPicoprecipitate may significantly enhance its utility for gene transfer to the airway …
Abstract
Adeno-associated virus (AAV) is inefficient at infecting differentiated airway epithelia because of a lack of receptors at the apical surface. We hypothesized that incorporation of AAV in a calcium phosphate coprecipitate would circumvent this barrier. Interestingly, coprecipitation of AAV type 2 improved gene transfer to differentiated human airway epithelia in vitro and to the mouse lung in vivo. These results suggest that delivery of AAV as a CaPicoprecipitate may significantly enhance its utility for gene transfer to the airway epithelia in vivo.
American Society for Microbiology