Cloning of a serotonin transporter affected by antidepressants

BJ Hoffman, E Mezey, MJ Brownstein - Science, 1991 - science.org
BJ Hoffman, E Mezey, MJ Brownstein
Science, 1991science.org
A complementary DNA clone for a serotonin (5HT) transporter has been isolated from rat
basophilic leukemia cells. The complementary DNA sequence predicts a 653-amino acid
protein with 12 to 13 putative transmembrane domains. The 5HT transporter has significant
homology to the γ-aminobutyric acid, dopamine, and norepinephrine transporters. Uptake by
CV-1 cells expressing the transporter complementary DNA resembles 5HT uptake by
platelets and brain synaptosomes; it is sensitive to antidepressants, amphetamine …
A complementary DNA clone for a serotonin (5HT) transporter has been isolated from rat basophilic leukemia cells. The complementary DNA sequence predicts a 653-amino acid protein with 12 to 13 putative transmembrane domains. The 5HT transporter has significant homology to the γ-aminobutyric acid, dopamine, and norepinephrine transporters. Uptake by CV-1 cells expressing the transporter complementary DNA resembles 5HT uptake by platelets and brain synaptosomes; it is sensitive to antidepressants, amphetamine derivatives, and cocaine.
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