Human eosinophils, stimulated with phorbol myristate acetate, were found to produce 1268
nm chemiluminescence characteristic of singlet oxygen. Singlet oxygen generation required
the presence of bromide ion. A bromide ion concentration of 100 microM, comparable to the
total bromine content of whole blood, was sufficient for the eosinophils to generate
measurable amounts of singlet oxygen. For the conditions used (10 (7) cells/ml and 10
micrograms/ml phorbol myristate acetate), the duration of the singlet oxygen generation was …