The injurious effect of eosinophil peroxidase, hydrogen peroxide, and halides on pneumocytes in vitro
JM Agosti, LC Altman, GH Ayars, DA Loegering… - Journal of allergy and …, 1987 - Elsevier
Recent data suggest that eosinophils may cause lung injury. To determine if the eosinophil
peroxidase (EPO)-hydrogen peroxide (H 2 O 2)-halide system could mediate this injury, we
added human EPO, H 2 O 2 (or glucose and glucose oxidase as a continuous source of H 2
O 2), and various halides to monolayers of 51 Cr-labeled human A549 and rat type II
pneumocytes. Cell lysis was measured as soluble 51 Cr release. In initial experiments, EPO
in solution did not induce lysis under these conditions. Therefore, in subsequent …
peroxidase (EPO)-hydrogen peroxide (H 2 O 2)-halide system could mediate this injury, we
added human EPO, H 2 O 2 (or glucose and glucose oxidase as a continuous source of H 2
O 2), and various halides to monolayers of 51 Cr-labeled human A549 and rat type II
pneumocytes. Cell lysis was measured as soluble 51 Cr release. In initial experiments, EPO
in solution did not induce lysis under these conditions. Therefore, in subsequent …