Guided selection of antibody fragments specific for human interferon γ receptor 1 from a human VH-and VL-gene repertoire

H Watzka, K Pfizenmaier, D Moosmayer - Immunotechnology, 1998 - Elsevier
Objective: The guided selection strategy for isolation of human antibody (Ab) fragments
specific for human interferon γ receptor 1 (IFNGR-1) from a cloned Ab VH and VL repertoire
has been investigated. In order to identify recombinant Abs binding to soluble antigen, a
novel method termed affinity sedimentation was introduced here. Results and conclusions:
The VH region of murine monoclonal Ab (IRγ-1) against human IFNGR-1 was combined with
human VL repertoire and used for selection of human VL regions. One of these human VL …