The influence of medium components on Cu2+-dependent oxidation of low-density lipoproteins and its sensitivity to superoxide dismutase
CE Thomas - Biochimica et Biophysica Acta (BBA)-Lipids and Lipid …, 1992 - Elsevier
The extent of in vitro Cu 2+-dependent oxidation of low-density lipoproteins (LDL) has been
reported to vary widely depending upon reaction conditions. In this study, the effect of
proteins and amino acids on Cu 2+-induced LDL oxidation was examined. Treatment of LDL
with 5 μM CuSO 4 for 18 h in either phosphate-buffered saline (PBS) or Ham's F-10 medium
resulted in extensive oxidation as determined by the content of thiobarbituric acid reactive
substances (TBARS) and by increased lipoprotein electronegativity. In PBS, oxidation was …
reported to vary widely depending upon reaction conditions. In this study, the effect of
proteins and amino acids on Cu 2+-induced LDL oxidation was examined. Treatment of LDL
with 5 μM CuSO 4 for 18 h in either phosphate-buffered saline (PBS) or Ham's F-10 medium
resulted in extensive oxidation as determined by the content of thiobarbituric acid reactive
substances (TBARS) and by increased lipoprotein electronegativity. In PBS, oxidation was …