Osteocalcin: isolation, characterization, and detection
CM Gundberg, PV Hauschka, JB Lian, PM Gallop - Methods in enzymology, 1984 - Elsevier
Publisher Summary This chapter discusses the isolation, characterization, and detection of
osteocalcin. Osteocalcin is a small protein (5800 daltons) comprising 10-20% of the
noncollagenous protein in the bone. Because osteocalcin is tightly adsorbed to the
hydroxyapatite mineral phase of the bone, thorough extraction is best achieved by
dissolving the mineral of finely pulverized bone. Hydroxyapatite is soluble in neutral 0.5 M
ethylenediaminetetraacetic acid (EDTA), as well as in a variety of mineral and organic acids …
osteocalcin. Osteocalcin is a small protein (5800 daltons) comprising 10-20% of the
noncollagenous protein in the bone. Because osteocalcin is tightly adsorbed to the
hydroxyapatite mineral phase of the bone, thorough extraction is best achieved by
dissolving the mineral of finely pulverized bone. Hydroxyapatite is soluble in neutral 0.5 M
ethylenediaminetetraacetic acid (EDTA), as well as in a variety of mineral and organic acids …