Activation of the atrial natriuretic peptide (ANP) gene is regarded as one of the earliest and
most reliable markers of hypertrophy in the ventricular cardiac myocyte. We have examined
the role of the nonreceptor tyrosine kinases in the signaling mechanism (s) leading to
hypertrophy using human ANP gene promoter activity as a marker. Endothelin (ET), a well
known hypertrophic agonist, increased activity of c-Src, c-Yes, and Fyn within minutes and
promoted a selective redistribution of each of these kinases within the cell. Overexpression …