Effect of insulin-like growth factor binding protein-1 on integrin signalling and the induction of apoptosis in human breast cancer cells
CM Perks, PV Newcomb… - Journal of molecular …, 1999 - jme.bioscientifica.com
Journal of molecular endocrinology, 1999•jme.bioscientifica.com
Interaction of epithelial cells with the extracellular matrix is mediated through integrin
receptors, which transmit signals regulating cell growth, differentiation and death.
Occupation of these receptors, via Arg-Gly-Asp (RGD) recognition sequences, leads to
activation of focal adhesion kinase (FAK). We treated human breast cancer cell lines with
RGD-containing peptides, which can disrupt integrin attachment, and investigated
alterations in FAK phosphorylation, cell detachment and death. Cells grown in vitro were …
receptors, which transmit signals regulating cell growth, differentiation and death.
Occupation of these receptors, via Arg-Gly-Asp (RGD) recognition sequences, leads to
activation of focal adhesion kinase (FAK). We treated human breast cancer cell lines with
RGD-containing peptides, which can disrupt integrin attachment, and investigated
alterations in FAK phosphorylation, cell detachment and death. Cells grown in vitro were …
Abstract
Interaction of epithelial cells with the extracellular matrix is mediated through integrin receptors, which transmit signals regulating cell growth, differentiation and death. Occupation of these receptors, via Arg-Gly-Asp (RGD) recognition sequences, leads to activation of focal adhesion kinase (FAK).
We treated human breast cancer cell lines with RGD-containing peptides, which can disrupt integrin attachment, and investigated alterations in FAK phosphorylation, cell detachment and death. Cells grown in vitro were treated with insulin-like growth factor-binding protein-1 (IGFBP-1) and a small, synthetic RGD-containing peptide (Gly-Arg-Gly-Asp-Thr-Pro) and its negative control peptide RGE (Arg-Gly-Glu-Ser) for either 30 min
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