Human acyl-CoA: cholesterol acyltransferase-1 in the endoplasmic reticulum contains seven transmembrane domains
S Lin, D Cheng, MS Liu, J Chen, TY Chang - Journal of Biological …, 1999 - ASBMB
Acyl-CoA: cholesterol acyltransferase (ACAT) plays important roles in cellular cholesterol
homeostasis and is involved in atherosclerosis. ACAT-1 protein is located mainly in the ER.
The hydropathy plot suggests that ACAT-1 protein contains multiple transmembrane
segments. We inserted either the hemagglutinin tag or the HisT7 tag at various hydrophilic
regions within the human ACAT-1 protein and used immunofluorescence microscopy to
determine the topography of the tagged proteins expressed in mutant Chinese hamster …
homeostasis and is involved in atherosclerosis. ACAT-1 protein is located mainly in the ER.
The hydropathy plot suggests that ACAT-1 protein contains multiple transmembrane
segments. We inserted either the hemagglutinin tag or the HisT7 tag at various hydrophilic
regions within the human ACAT-1 protein and used immunofluorescence microscopy to
determine the topography of the tagged proteins expressed in mutant Chinese hamster …
