Characterization of an Krox-24/Egr-1-responsive element in the human tumor necrosis factor promoter
B Krämer, A Meichle, G Hensel, P Charnay… - Biochimica et Biophysica …, 1994 - Elsevier
We have analyzed in various human leukemic cell lines a previously unrecognized region
within the human TNF gene promoter that contains the sequence motif 5′-
CCGCCCCCGCG-3′. This GC-rich sequence maps to bps− 170 and− 160 of the TNF
gene. Electrophoretic mobility shift assays (EMSA) combined with methylation interference
analysis revealed the binding of two distinct proteins with overlapping recognition sites.
Supershift assays identified the constitutive transcription factor Sp1 and the immediate-early …
within the human TNF gene promoter that contains the sequence motif 5′-
CCGCCCCCGCG-3′. This GC-rich sequence maps to bps− 170 and− 160 of the TNF
gene. Electrophoretic mobility shift assays (EMSA) combined with methylation interference
analysis revealed the binding of two distinct proteins with overlapping recognition sites.
Supershift assays identified the constitutive transcription factor Sp1 and the immediate-early …