Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies
CB Laurell - Analytical biochemistry, 1966 - Elsevier
CB Laurell
Analytical biochemistry, 1966•ElsevierA method is deseribed for quantitative analysis of proteins with a charge differing from that of
the bulk of the immunoglobulins. The method utilizes the difference between the rate of
electrophoretic migration of proteins and of their antibody complexes in agarose gel. The
method has an error of a few per cent; it is rapid, suitable for serial analysis, and requires 2
to 0.5 μg protein antigen. The amount of antiserum needed is slightly less than with the
radial immunodiffusion techniques.
the bulk of the immunoglobulins. The method utilizes the difference between the rate of
electrophoretic migration of proteins and of their antibody complexes in agarose gel. The
method has an error of a few per cent; it is rapid, suitable for serial analysis, and requires 2
to 0.5 μg protein antigen. The amount of antiserum needed is slightly less than with the
radial immunodiffusion techniques.
A method is deseribed for quantitative analysis of proteins with a charge differing from that of the bulk of the immunoglobulins. The method utilizes the difference between the rate of electrophoretic migration of proteins and of their antibody complexes in agarose gel. The method has an error of a few per cent; it is rapid, suitable for serial analysis, and requires 2 to 0.5 μg protein antigen. The amount of antiserum needed is slightly less than with the radial immunodiffusion techniques.
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