Cholesteryl ester transfer protein gene expression during differentiation of human preadipocytes to adipocytes in primary culture
B Gauthier, M Robb, R McPherson - Atherosclerosis, 1999 - Elsevier
The expression pattern of the CETP gene in relationship to that of LPL, adipsin, PPARγ,
C/EBPα, ADD1/SREBP1 and actin was examined by RT-PCR during differentiation of
human fibroblastic preadipocytes to adipocytes in primary culture. Preadipocytes were
isolated from subcutaneous fat obtained from healthy female subjects undergoing mammary
reduction procedures, and induced to differentiate in culture. Morphologically, adipogenesis
was confirmed by the accumulation of lipid droplets in cells. We show that the gene …
C/EBPα, ADD1/SREBP1 and actin was examined by RT-PCR during differentiation of
human fibroblastic preadipocytes to adipocytes in primary culture. Preadipocytes were
isolated from subcutaneous fat obtained from healthy female subjects undergoing mammary
reduction procedures, and induced to differentiate in culture. Morphologically, adipogenesis
was confirmed by the accumulation of lipid droplets in cells. We show that the gene …