DNA binding and IκB inhibition of the cloned p65 subunit of NF-κB, a rel-related polypeptide

GP Nolan, S Ghosh, HC Liou, P Tempst, D Baltimore - Cell, 1991 - cell.com
GP Nolan, S Ghosh, HC Liou, P Tempst, D Baltimore
Cell, 1991cell.com
The sequence and biochemical properties of the prod-Nuct of the cloned cDNA for the~ 65
subunit of nuclear, factor KB (NF-KB) have been determined. The cDNA has an open
reading frame of 549 amino acids capable of encoding a 60 kd protein. NF-KB~ 65 contains
an amino-terminal region of 320 amino acids with extensive similarity to the oncogene c-rel
and lesser similarity to NF-KB~ 50. In vitro translated~ 65 forms a DNA-binding complex with
NF-KB~ 50, and the binding of this complex can be specifically inhibited by purified IKB …
Summary
The sequence and biochemical properties of the prod-Nuct of the cloned cDNA for the~ 65 subunit of nuclear, factor KB (NF-KB) have been determined. The cDNA has an open reading frame of 549 amino acids capable of encoding a 60 kd protein. NF-KB~ 65 contains an amino-terminal region of 320 amino acids with extensive similarity to the oncogene c-rel and lesser similarity to NF-KB~ 50. In vitro translated~ 65 forms a DNA-binding complex with NF-KB~ 50, and the binding of this complex can be specifically inhibited by purified IKB. Progressive carboxy-terminal deletions of~ 65 show that, contrary to previous assumptions,~ 65 does include a DNA-binding domain that in vivo might become activated only through hetero-oligomerization with~ 50. DNA binding by truncated~ 65 is inhibited by IKB, thus mapping the IKB interaction domain to the rel-homologous region and suggesting that IKB exerts its inhibitory effect upon NF-KB primarily through interaction with~ 65.
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