[HTML][HTML] Bidirectional water fluxes and specificity for small hydrophilic molecules in aquaporins 0–5
AK Meinild, DA Klaerke, T Zeuthen - Journal of Biological Chemistry, 1998 - ASBMB
The dimensions of the aqueous pore in aquaporins (AQP) 0, 1, 2, 3, 4, and 5 expressed in
Xenopus laevisoocytes were probed by comparing the ability of various solutes to generate
osmotic flow. By improved techniques, volume flows were determined from initial rates of
changes. Identical values for the osmotic water permeability (L p) were obtained in swelling
as in shrinkage experiments demonstrating, for the first time, that aquaporins are
bidirectional. The reflection coefficients (ς) of urea, glycerol, acetamide, and formamide at …
Xenopus laevisoocytes were probed by comparing the ability of various solutes to generate
osmotic flow. By improved techniques, volume flows were determined from initial rates of
changes. Identical values for the osmotic water permeability (L p) were obtained in swelling
as in shrinkage experiments demonstrating, for the first time, that aquaporins are
bidirectional. The reflection coefficients (ς) of urea, glycerol, acetamide, and formamide at …