[HTML][HTML] Water and glycerol permeabilities of aquaporins 1–5 and MIP determined quantitatively by expression of epitope-tagged constructs inXenopus oocytes
B Yang, AS Verkman - Journal of Biological Chemistry, 1997 - ASBMB
The goal of this study was to compare single channel water and glycerol permeabilities of
mammalian aquaporins (AQP) 1–5 and the major intrinsic protein of lens fiber (MIP). Each of
the six cloned cDNAs from rat was left untagged or was epitope-tagged with c-Myc or FLAG
at either the N or C terminus so that results would not depend on epitope identity or location.
The constructs were expressed in Xenopus oocytes for measurement of osmotic water
permeability (P f),[3 H] glycerol uptake, and protein expression. Each of the 30 epitope …
mammalian aquaporins (AQP) 1–5 and the major intrinsic protein of lens fiber (MIP). Each of
the six cloned cDNAs from rat was left untagged or was epitope-tagged with c-Myc or FLAG
at either the N or C terminus so that results would not depend on epitope identity or location.
The constructs were expressed in Xenopus oocytes for measurement of osmotic water
permeability (P f),[3 H] glycerol uptake, and protein expression. Each of the 30 epitope …