[HTML][HTML] P2X1 purinoceptor in human platelets: molecular cloning and functional characterization after heterologous expression

B Sun, J Li, K Okahara, J Kambayashi - Journal of Biological Chemistry, 1998 - ASBMB
ADP is an important physiological platelet agonist. The molecular identity of the ADP
receptor (s) in human platelets, however, is still unclear. Although P2T purinoceptor was
believed to be the ligand-gated cation channel for ADP in human platelets, recent patch
clamp studies now suggest it is P2X 1 type. In the present study, we have cloned a cDNA
encoding a P2X 1 purinoceptor from human platelets using degenerate reverse transcription
and polymerase chain reaction. Northern blotting with a P2X 1-specific probe revealed a …