Activation of the Na+/H+ antiporter during cell volume regulation. Evidence for a phosphorylation-independent mechanism.
S Grinstein, M Woodside, C Sardet… - Journal of Biological …, 1992 - ASBMB
A variety of cell types regulate their volume in anisotonic media by stimulating Na+/H+
exchange. Like growth factors, osmotic challenge activates the Na+/H+ antiport by
increasing its sensitivity to intracellular [H+]. To investigate the molecular mechanism
underlying this shift in pH sensitivity, the antiporter of 32P-labeled human bladder carcinoma
cells and of Chinese hamster ovary cells was immunoprecipitated using antibodies raised
against the cytosolic domain of the NHE-1 isoform of the Na+/H+ exchanger. Unlike the …
exchange. Like growth factors, osmotic challenge activates the Na+/H+ antiport by
increasing its sensitivity to intracellular [H+]. To investigate the molecular mechanism
underlying this shift in pH sensitivity, the antiporter of 32P-labeled human bladder carcinoma
cells and of Chinese hamster ovary cells was immunoprecipitated using antibodies raised
against the cytosolic domain of the NHE-1 isoform of the Na+/H+ exchanger. Unlike the …