[HTML][HTML] Activation of Na+/H+ exchange is required for regulatory volume decrease after modest “physiological” volume increases in jejunal villus epithelial cells
RJ MacLeod, JR Hamilton - Journal of Biological Chemistry, 1996 - ASBMB
Epithelial cell volume increases that occur because of the uptake of Na+-cotransported
solutes or hypotonic dilution are followed by a regulatory volume decrease (RVD) due to the
activation of K+ and Cl− channels. We studied the relationship of Na+/H+ exchange (NHE)
to this RVD in suspended guinea pig jejunal villus cells, using electronic sizing to measure
cell volume changes and fluorescent spectroscopy of cells loaded with 2′, 7′-bis
(carboxyethyl)-5 (6)-carboxyfluorescein to monitor intracellular pH (pH i). When the volume …
solutes or hypotonic dilution are followed by a regulatory volume decrease (RVD) due to the
activation of K+ and Cl− channels. We studied the relationship of Na+/H+ exchange (NHE)
to this RVD in suspended guinea pig jejunal villus cells, using electronic sizing to measure
cell volume changes and fluorescent spectroscopy of cells loaded with 2′, 7′-bis
(carboxyethyl)-5 (6)-carboxyfluorescein to monitor intracellular pH (pH i). When the volume …