Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine-dependent cell lines
TM Buttke, JA McCubrey, TC Owen - Journal of immunological methods, 1993 - Elsevier
A new tetrazolium compound, MTS (3-(4, 5-dimethylthiazol-2-yl)-5-(3-
carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt), has recently been
described which in the presence of phenazine methosulfate (PMS) is reduced by living cells
to yield a formazan product that can be assayed colorimetrically. An important advantage of
MTS/PMS over other tetrazolium dyes (eg, MTT) is the aqueous solubility of the reduced
formazan product which eliminates the need for detergent solubilization or organic solvent …
carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt), has recently been
described which in the presence of phenazine methosulfate (PMS) is reduced by living cells
to yield a formazan product that can be assayed colorimetrically. An important advantage of
MTS/PMS over other tetrazolium dyes (eg, MTT) is the aqueous solubility of the reduced
formazan product which eliminates the need for detergent solubilization or organic solvent …