[HTML][HTML] The N-end rule pathway catalyzes a major fraction of the protein degradation in skeletal muscle
V Solomon, SH Lecker, AL Goldberg - Journal of Biological Chemistry, 1998 - ASBMB
In skeletal muscle, overall protein degradation involves the ubiquitin-proteasome system.
One property of a protein that leads to rapid ubiquitin-dependent degradation is the
presence of a basic, acidic, or bulky hydrophobic residue at its N terminus. However, in
normal cells, substrates for this N-end rule pathway, which involves ubiquitin carrier protein
(E2) E2 14k and ubiquitin-protein ligase (E3) E3α, have remained unclear. Surprisingly, in
soluble extracts of rabbit muscle, we found that competitive inhibitors of E3α markedly …
One property of a protein that leads to rapid ubiquitin-dependent degradation is the
presence of a basic, acidic, or bulky hydrophobic residue at its N terminus. However, in
normal cells, substrates for this N-end rule pathway, which involves ubiquitin carrier protein
(E2) E2 14k and ubiquitin-protein ligase (E3) E3α, have remained unclear. Surprisingly, in
soluble extracts of rabbit muscle, we found that competitive inhibitors of E3α markedly …