[HTML][HTML] Membrane trafficking of the cystic fibrosis gene product, cystic fibrosis transmembrane conductance regulator, tagged with green fluorescent protein in Madin …
BD Moyer, J Loffing, EM Schwiebert… - Journal of Biological …, 1998 - ASBMB
The mechanism by which cAMP stimulates cystic fibrosis transmembrane conductance
regulator (CFTR)-mediated chloride (Cl−) secretion is cell type-specific. By using Madin-
Darby canine kidney (MDCK) type I epithelial cells as a model, we tested the hypothesis that
cAMP stimulates Cl− secretion by stimulating CFTR Cl− channel trafficking from an
intracellular pool to the apical plasma membrane. To this end, we generated a green
fluorescent protein (GFP)-CFTR expression vector in which GFP was linked to the N …
regulator (CFTR)-mediated chloride (Cl−) secretion is cell type-specific. By using Madin-
Darby canine kidney (MDCK) type I epithelial cells as a model, we tested the hypothesis that
cAMP stimulates Cl− secretion by stimulating CFTR Cl− channel trafficking from an
intracellular pool to the apical plasma membrane. To this end, we generated a green
fluorescent protein (GFP)-CFTR expression vector in which GFP was linked to the N …