[HTML][HTML] The p38/reactivating kinase mitogen-activated protein kinase cascade mediates the activation of the transcription factor insulin upstream factor 1 and insulin …

WM Macfarlane, SB Smith, RFL James… - Journal of Biological …, 1997 - ASBMB
Insulin upstream factor 1 (IUF1), a transcription factor present in pancreatic β-cells, binds to
the sequence C (C/T) TAATG present at several sites within the human insulin promoter.
Here we isolated and sequenced cDNA encoding human IUF1 and exploited it to identify the
signal transduction pathway by which glucose triggers its activation. In human islets, or in
the mouse β-cell line MIN6, high glucose induced the binding of IUF1 to DNA, an effect
mimicked by serine/threonine phosphatase inhibitors, indicating that DNA binding was …