Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins

MB Lawrence, TA Springer - Cell, 1991 - cell.com
Cell, 1991cell.com
Rolling of leukocytes on vascular endotheliai cells, an early event in inflammation, can be
reproduced in vitro on artificial lipid bilayers containing purified CD62, a seiectln also named
PADGEM and GMP-140 that is inducible on endothelial cells. Neutrophils roll on this
selectin under flow conditions similar to those found in postcapillary venules. Adhesion of
resting or activated neutrophils through the integrins LFA-1 and Mac-l to ICAM-in a lipid
bilayer does not occur at physiologic shear stresses; however, static incubation of activated …
Summary
Rolling of leukocytes on vascular endotheliai cells, an early event in inflammation, can be reproduced in vitro on artificial lipid bilayers containing purified CD62, a seiectln also named PADGEM and GMP-140 that is inducible on endothelial cells. Neutrophils roll on this selectin under flow conditions similar to those found in postcapillary venules. Adhesion of resting or activated neutrophils through the integrins LFA-1 and Mac-l to ICAM-in a lipid bilayer does not occur at physiologic shear stresses; however, static incubation of activated neutrophils allows development of adhesion that is greater than 1 OO-fold more shear resistant than found on CD62. Addition of a chemoattractant to activate LFA-1 and Mac-l results in the arrest of neutrophils rolling on bilayers containing both CD62 and ICAY-1. Thus, at physiologic shear stress, roiling on a selectin is a prerequisite for activation-induced adhesion strengthening through integrins.
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