Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase

DS Bredt, PM Hwang, CE Glatt, C Lowenstein… - Nature, 1991 - nature.com
DS Bredt, PM Hwang, CE Glatt, C Lowenstein, RR Reed, SH Snyder
Nature, 1991nature.com
Nitric oxide is a messenger molecule, mediating the effect of endothelium-derived relaxing
factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in
neuronal communication in the brain. Cloning of a complementary DNA for brain nitric oxide
synthase reveals recognition sites for NADPH, FAD, flavin mononucleotide and calmodulin
as well as phosphorylation sites, indicating that the synthase is regulated by many different
factors. The only known mammalian enzyme with close homology is cytochrome P-450 …
Abstract
Nitric oxide is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. Cloning of a complementary DNA for brain nitric oxide synthase reveals recognition sites for NADPH, FAD, flavin mononucleotide and calmodulin as well as phosphorylation sites, indicating that the synthase is regulated by many different factors. The only known mammalian enzyme with close homology is cytochrome P-450 reductase.
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