[HTML][HTML] Molecular cloning of a novel T cell-directed CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector
T Imai, T Yoshida, M Baba, M Nishimura… - Journal of Biological …, 1996 - ASBMB
Precursors of most secreted and cell surface molecules carry signal sequences at their
amino termini. Here we describe an efficient signal sequence trap method and isolation of a
novel CC chemokine. An expression library was constructed by inserting 5′ portion-
enriched cDNAs from phytohemagglutinin-stimulated peripheral blood mononuclear cells
into upstream of signal sequence-deleted CD4 cDNA in an Epstein-Barr virus shuttle vector.
After electroporation into Raji cells, CD4 antigen-positive cells were enriched by repeated …
amino termini. Here we describe an efficient signal sequence trap method and isolation of a
novel CC chemokine. An expression library was constructed by inserting 5′ portion-
enriched cDNAs from phytohemagglutinin-stimulated peripheral blood mononuclear cells
into upstream of signal sequence-deleted CD4 cDNA in an Epstein-Barr virus shuttle vector.
After electroporation into Raji cells, CD4 antigen-positive cells were enriched by repeated …