[HTML][HTML] Interleukin 8 Is Induced by Cholesterol Loading of Macrophages and Expressed by Macrophage Foam Cells in Human Atheroma (∗)
In order to identify novel genes expressed in macrophage-derived foam cells, we used a
multigene assay to examine the expression of genes in control versus cholesterol-loaded
macrophages. We compared THP-1 macrophages incubated with or without acetylated LDL
(acLDL)±acyl-CoA: cholesterol O-acyltransferase (ACAT) inhibitor (compound 58035) for 20
h and assessed changes in mRNA of chemokines, growth factors, interleukins, and
adhesion molecules. Among 49 genes examined, an increase in mRNA was observed only …
multigene assay to examine the expression of genes in control versus cholesterol-loaded
macrophages. We compared THP-1 macrophages incubated with or without acetylated LDL
(acLDL)±acyl-CoA: cholesterol O-acyltransferase (ACAT) inhibitor (compound 58035) for 20
h and assessed changes in mRNA of chemokines, growth factors, interleukins, and
adhesion molecules. Among 49 genes examined, an increase in mRNA was observed only …