Human recombinant interleukin (IL) induced migration across polycarbonate filters of human peripheral blood eosinophils. The contribution of chemotaxis vs. chemokinesis was investigated using a checkerboard design with both polycarbonate and nitrocellulose filters. When different cytokine concentrations were seeded above and below the filter, maximal induction of migration required a positive concentration gradient between the lower and upper compartments of the chamber, though some gradient‐independent augmentation of migration occurred. These results indicate that induction of eosinophil migration across filter involves actual chemotaxis. The effect of IL 5 was selective for eosinophils with no effect on neutrophils and monocytes. Conversely, granulocyte‐macrophage colony‐stimulating factor elicited migration of both eosinophils and neutrophils. Thus, human IL 5 is a potent and selective chemoattractant for human eosinophils. Eosinophils are selectively localized in tissues under a variety of physiological and pathological conditions. Locally produced IL 5 may play a role in the selective recruitment of eosinophils from the blood compartment.