[HTML][HTML] Mutational analysis of cysteine-rich domains of the Epithelium Sodium Channel (ENaC): identification of cysteines essential for channel expression at the cell …
D Firsov, M Robert-Nicoud, S Gruender, L Schild… - Journal of Biological …, 1999 - ASBMB
One of the characteristic features of the structure of the epithelial sodium channel family
(ENaC) is the presence of two highly conserved cysteine-rich domains (CRD1 and CRD2) in
the large extracellular loops of the proteins. We have studied the role of CRDs in the
functional expression of rat αβγ ENaC subunits by systematically mutating cysteine residues
(singly or in combinations) into either serine or alanine. In the Xenopusoocyte expression
system, mutations of two cysteines in CRD1 of α, β, or γ ENaC subunits led to a temperature …
(ENaC) is the presence of two highly conserved cysteine-rich domains (CRD1 and CRD2) in
the large extracellular loops of the proteins. We have studied the role of CRDs in the
functional expression of rat αβγ ENaC subunits by systematically mutating cysteine residues
(singly or in combinations) into either serine or alanine. In the Xenopusoocyte expression
system, mutations of two cysteines in CRD1 of α, β, or γ ENaC subunits led to a temperature …