[HTML][HTML] Molecular identification of a novel fibrinogen binding site on the first domain of ICAM-1 regulating leukocyte-endothelium bridging
A Duperray, LR Languino, J Plescia… - Journal of Biological …, 1997 - ASBMB
Binding of fibrinogen to intercellular adhesion molecule 1 (ICAM-1) enhances leukocyte
adhesion to endothelium by acting as a bridging molecule between the two cell types. Here,
a panel of four monoclonal antibodies (mAbs) to ICAM-1 was used to dissect the structure-
function requirements of this recognition. All four mAbs bound to ICAM-1 transfectants and
immunoprecipitated and immunoblotted ICAM-1 from detergent-solubilized JY lymphocyte
extracts. Functionally, mAbs 1G12 and 2D5 inhibited binding of 125 I-fibrinogen to ICAM-1 …
adhesion to endothelium by acting as a bridging molecule between the two cell types. Here,
a panel of four monoclonal antibodies (mAbs) to ICAM-1 was used to dissect the structure-
function requirements of this recognition. All four mAbs bound to ICAM-1 transfectants and
immunoprecipitated and immunoblotted ICAM-1 from detergent-solubilized JY lymphocyte
extracts. Functionally, mAbs 1G12 and 2D5 inhibited binding of 125 I-fibrinogen to ICAM-1 …