Altered metabolism of thrombospondin by Chinese hamster ovary cells defective in glycosaminoglycan synthesis.
JE Murphy-Ullrich, LG Westrick, JD Esko… - Journal of Biological …, 1988 - ASBMB
We examined the ability of Chinese hamster ovary (CHO) cell mutants defective in
glycosaminoglycan synthesis to metabolize 125I-labeled thrombospondin (TSP). Wild type
CHO cells bound and degraded 125I-TSP with kinetics similar to those reported for
endothelial cells. Both binding and degradation were saturable (half-saturation at 20
micrograms/ml). When the concentration of labeled TSP was 1-5 micrograms/ml, mutant
745, defective in xylosyltransferase, and mutant 761, defective in galactosyltransferase I …
glycosaminoglycan synthesis to metabolize 125I-labeled thrombospondin (TSP). Wild type
CHO cells bound and degraded 125I-TSP with kinetics similar to those reported for
endothelial cells. Both binding and degradation were saturable (half-saturation at 20
micrograms/ml). When the concentration of labeled TSP was 1-5 micrograms/ml, mutant
745, defective in xylosyltransferase, and mutant 761, defective in galactosyltransferase I …