Natural forms of shed tumor gangliosides
Y Kong, R Li, S Ladisch - Biochimica et Biophysica Acta (BBA)-Lipids and …, 1998 - Elsevier
Gangliosides shed by tumor cells are immunosuppressive molecules, but the mechanisms
of shedding are poorly understood. We therefore conducted a comprehensive study of
shedding to identify the natural forms of shed gangliosides. By chemical detection and mass
spectrometric analysis of the gangliosides of YAC-1 murine lymphoma cells, we first
confirmed that all major ganglioside species are released. Then, by the combination of
metabolic and cell surface radiolabeling, we further demonstrated that gangliosides are …
of shedding are poorly understood. We therefore conducted a comprehensive study of
shedding to identify the natural forms of shed gangliosides. By chemical detection and mass
spectrometric analysis of the gangliosides of YAC-1 murine lymphoma cells, we first
confirmed that all major ganglioside species are released. Then, by the combination of
metabolic and cell surface radiolabeling, we further demonstrated that gangliosides are …