Sensitive and specific immunoassays to detect rabbit IL-8 and MCP-1: cytokines that mediate leukocyte recruitment to the lungs
O Kajikawa, RB Goodman, MC Johnson II… - Journal of …, 1996 - Elsevier
The α-and β-chemokines such as IL-8 and MCP-1 direct the recruitment of neutrophils and
monocytes into the lungs and other tissues. In order to study the roles of IL-8 and MCP-1 in
animal models, specific reagents are required that provide accurate measurements of these
cytokines in biological fluids. Here we describe the development of sensitive and specific
immunoassays for rabbit IL-8 and rabbit MCP-1, and the validation of these assays in rabbit
plasma and bronchoalveolar lavage fluid. The sensitivity of each assay is 0.25 ng/ml for IL-8 …
monocytes into the lungs and other tissues. In order to study the roles of IL-8 and MCP-1 in
animal models, specific reagents are required that provide accurate measurements of these
cytokines in biological fluids. Here we describe the development of sensitive and specific
immunoassays for rabbit IL-8 and rabbit MCP-1, and the validation of these assays in rabbit
plasma and bronchoalveolar lavage fluid. The sensitivity of each assay is 0.25 ng/ml for IL-8 …