[HTML][HTML] Protein kinase C-ζ as a downstream effector of phosphatidylinositol 3-kinase during insulin stimulation in rat adipocytes: potential role in glucose transport
ML Standaert, L Galloway, P Karnam… - Journal of Biological …, 1997 - ASBMB
Insulin provoked rapid increases in enzyme activity of immunoprecipitable protein kinase C-
ζ (PKC-ζ) in rat adipocytes. Concomitantly, insulin provoked increases in 32 P labeling of
PKC-ζ both in intact adipocytes and during in vitro assay of immunoprecipitated PKC-ζ; the
latter probably reflected autophosphorylation, as it was inhibited by the PKC-ζ
pseudosubstrate. Insulin-induced activation of immunoprecipitable PKC-ζ was inhibited by
LY294002 and wortmannin; this suggested dependence upon phosphatidylinositol (PI) 3 …
ζ (PKC-ζ) in rat adipocytes. Concomitantly, insulin provoked increases in 32 P labeling of
PKC-ζ both in intact adipocytes and during in vitro assay of immunoprecipitated PKC-ζ; the
latter probably reflected autophosphorylation, as it was inhibited by the PKC-ζ
pseudosubstrate. Insulin-induced activation of immunoprecipitable PKC-ζ was inhibited by
LY294002 and wortmannin; this suggested dependence upon phosphatidylinositol (PI) 3 …