Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody

PD Kwong, R Wyatt, J Robinson, RW Sweet, J Sodroski… - Nature, 1998 - nature.com
PD Kwong, R Wyatt, J Robinson, RW Sweet, J Sodroski, WA Hendrickson
Nature, 1998nature.com
The entry of human immunodeficiency virus (HIV) into cells requires the sequential
interaction of the viral exterior envelope glycoprotein, gp120, with the CD4 glycoprotein and
a chemokine receptor on the cell surface. These interactions initiate a fusion of the viral and
cellular membranes. Although gpl20 can elicit virus-neutralizing antibodies, HIV eludes the
immune system. We have solved the X-ray crystal structure at 2.5 Ĺ resolution of an HIV-1
gp120 core complexed with a two-domain fragment of human CD4 and an antigen-binding …
Abstract
The entry of human immunodeficiency virus (HIV) into cells requires the sequential interaction of the viral exterior envelope glycoprotein, gp120, with the CD4 glycoprotein and a chemokine receptor on the cell surface. These interactions initiate a fusion of the viral and cellular membranes. Although gpl20 can elicit virus-neutralizing antibodies, HIV eludes the immune system. We have solved the X-ray crystal structure at 2.5 Ĺ resolution of an HIV-1 gp120 core complexed with a two-domain fragment of human CD4 and an antigen-binding fragment of a neutralizing antibody that blocks chemokine-receptor binding. The structure reveals a cavity-laden CD4–gp120 interface, a conserved binding site for the chemokine receptor, evidence for a conformational change upon CD4 binding, the nature of a CD4-induced antibody epitope, and specific mechanisms for immune evasion. Our results provide a framework for understanding the complex biology of HIV entry into cells and should guide efforts to intervene.
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