[HTML][HTML] Casein kinase II associates with Egr-1 and acts as a negative modulator of its DNA binding and transcription activities in NIH 3T3 cells
N Jain, R Mahendran, R Philp, GR Guy, YH Tan… - Journal of Biological …, 1996 - ASBMB
Although the activation domains within early growth response gene protein 1 (Egr-1) have
been mapped, little is known of the kinases which phosphorylate Egr-1 and how
phosphorylation correlates with the transcriptional activity of Egr-1. In this study we report
that casein kinase II (CKII) co-immunoprecipitates with Egr-1 from NIH 3T3 cell lysates. The
association of Egr-1 and CKII requires the C terminus of Egr-1 and CKII phosphorylates Egr-
1 in vitro. The in vitro phosphorylation of Egr-1 by CKII and that induced by serum in vivo …
been mapped, little is known of the kinases which phosphorylate Egr-1 and how
phosphorylation correlates with the transcriptional activity of Egr-1. In this study we report
that casein kinase II (CKII) co-immunoprecipitates with Egr-1 from NIH 3T3 cell lysates. The
association of Egr-1 and CKII requires the C terminus of Egr-1 and CKII phosphorylates Egr-
1 in vitro. The in vitro phosphorylation of Egr-1 by CKII and that induced by serum in vivo …