Stimulated secretion of endothelial von Willebrand factor is accompanied by rapid redistribution to the cell surface of the intracellular granule membrane protein GMP …

R Hattori, KK Hamilton, RD Fugate, RP McEver… - Journal of Biological …, 1989 - ASBMB
We have examined the cell activation-dependent redistribution of the intracellular granule
membrane protein GMP-140 of human endothelial cells. By dual-label immunofluorescence,
the distribution of GMP-140 within cultured human umbilical vein endothelial cells was found
to coincide with the distribution of von Willebrand factor (vWF), suggesting that GMP-140 is
located in the membranes of vWF-containing storage granules. Stimulation of vWF secretion
resulted in an increase in GMP-140 on the cell surface, as detected by increased binding of …