Preparative procedures of cooling and re-warming increase leukocyte integrin expression and function on neotrophils
KD Forsyth, RJ Levinsky - Journal of immunological methods, 1990 - Elsevier
The majority of studies involving neutrophil integrin expression and function are performed
at physioligical temperatures subsequent to routine preparative procedures at 4° C. We
have shown that surface expression of the leukocyte integrin molecules on neutrophils is
increased by cooling and subsequently re-warming of neutrophils to 37° C when compared
with cells held at room temperature or 37° C. This increase in expression se secondary to
prior cooling of the neutrophils. There is an associated increase in function of these newly …
at physioligical temperatures subsequent to routine preparative procedures at 4° C. We
have shown that surface expression of the leukocyte integrin molecules on neutrophils is
increased by cooling and subsequently re-warming of neutrophils to 37° C when compared
with cells held at room temperature or 37° C. This increase in expression se secondary to
prior cooling of the neutrophils. There is an associated increase in function of these newly …
