Galanin is a peptide hormone widely expressed in the central nervous system and gastrointestinal (GI) tract. Within the GI tract galanin is present in enteric nerve terminals where it is known to modulate intestinal motility by altering smooth muscle contraction. Recent studies also show that galanin can alter intestinal short-circuit current (I _sc) but with differing results observed in rats, rabbits, guinea pigs, and pigs. In contrast, nothing is known about the ability of galanin to alter ion transport in human intestinal epithelial tissues. By RT-PCR, we determined that these tissues express only the galanin-1 receptor (Gal1-R) subtype. To evaluate Gal1-R pharmacology and physiology, we studied T84 cells. Gal1-R expressed by these cells bound galanin rapidly (half time 1–2 min) and with high affinity (inhibitor constant 0.7 ± 0.2 nM). T84 cells were then studied in a modified Ussing chamber and alterations inI _sc, a measure of all ion movement across the tissue, were determined. Maximal increases in I _sc were observed in a concentration-dependent manner around 2 min after stimulation with peptide, with 1 μM galanin causingI _sc to rise more than eightfold and return to baseline occurring within 10 min. The increase in galanin-inducedI _sc was shown by¹²⁵I efflux studies to be due to Cl⁻ secretion, which occurred independently of alterations in cAMP and phospholipase C. Rather, Cl⁻ secretion is mediated via a Ca²⁺-dependent, pertussis toxin-sensitive mechanism. These data suggest that galanin released by enteric nerves may act as a secretagogue in the human colon by activating Gal1-R.