A role for interleukin-1 in epidermal differentiation: regulation by expression of functional versus decoy receptors

MS Eller, M Yaar, K Ostrom… - Journal of cell …, 1995 - journals.biologists.com
MS Eller, M Yaar, K Ostrom, DD Harkness, BA Gilchrest
Journal of cell science, 1995journals.biologists.com
Although human epidermis contains levels of interleukin-1 (IL-1) up to 100 times higher than
other tissues, the role of this cytokine in epidermal biology is unknown. Here, we show that
interleukin-1 regulates the expression of mRNAs for two proteins associated with the
differentiated phenotype of human keratinocytes, cellular retinoic acidbinding protein type II
(CRABP II) and small, proline rich protein 1 (SPRR1). The ability of IL-1 to induce these
transcripts correlates directly with keratinocyte expression of the IL-1 receptor type I (IL-1 RI) …
Abstract
Although human epidermis contains levels of interleukin-1 (IL-1) up to 100 times higher than other tissues, the role of this cytokine in epidermal biology is unknown. Here, we show that interleukin-1 regulates the expression of mRNAs for two proteins associated with the differentiated phenotype of human keratinocytes, cellular retinoic acidbinding protein type II (CRABP II) and small, proline rich protein 1 (SPRR1). The ability of IL-1 to induce these transcripts correlates directly with keratinocyte expression of the IL-1 receptor type I (IL-1 RI) during differentiation and inversely with the expression of the type II IL-1 receptor (IL-1 RII), shown in other cell types to be a non-functional, decoy receptor. Furthermore, addition to ker-atinocyte cultures of an IL-1 RI-blocking, but not an IL-1 RII-blocking, antibody reduces the level of CRABP II and SPRR1 mRNAs in these cells. These data suggest that epidermal IL-1 functions to promote keratinocyte differentiation and that a change in the IL-1 receptor profile of these cells initiates this IL-1 response through a relative enhanced expression of functional IL-1 receptors.
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