Proteinase-induced formation of focal tight junctions in HT 29 adenocarcinoma cells does not require extracellular calcium
O Faff, E Cohen, A Bacher, Y Ben-Shaul - Biochimica et Biophysica Acta …, 1987 - Elsevier
O Faff, E Cohen, A Bacher, Y Ben-Shaul
Biochimica et Biophysica Acta (BBA)-Biomembranes, 1987•ElsevierThe human adenocarcinoma cell line HT 29 grows virtually without tight junctions, but the
formation of focal tight junctions can be induced by brief treatment with proteinases. The
freeze-fracture morphology of proteinase-induced tight juntions is not affected by treatment
with EGTA or EDTA over a period of 30 min. The induction of tight junctions by trypsin or
pronase can proceed in the presence of 3 mM EGTA or EDTA. Neither the formation nor the
structure and complexity of the induced tight junctions is affected by the chelators. It follows …
formation of focal tight junctions can be induced by brief treatment with proteinases. The
freeze-fracture morphology of proteinase-induced tight juntions is not affected by treatment
with EGTA or EDTA over a period of 30 min. The induction of tight junctions by trypsin or
pronase can proceed in the presence of 3 mM EGTA or EDTA. Neither the formation nor the
structure and complexity of the induced tight junctions is affected by the chelators. It follows …
Abstract
The human adenocarcinoma cell line HT 29 grows virtually without tight junctions, but the formation of focal tight junctions can be induced by brief treatment with proteinases. The freeze-fracture morphology of proteinase-induced tight juntions is not affected by treatment with EGTA or EDTA over a period of 30 min. The induction of tight junctions by trypsin or pronase can proceed in the presence of 3 mM EGTA or EDTA. Neither the formation nor the structure and complexity of the induced tight junctions is affected by the chelators. It follows that no extracellular divalent cations are required for the induced formation and the structural integrity of focal tight junctions in HT 29 cells.
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