Vitamin D receptors in breast cancer cells
RR Buras, LM Schumaker, F Davoodi… - Breast cancer research …, 1994 - Springer
RR Buras, LM Schumaker, F Davoodi, RV Brenner, M Shabahang, RJ Nauta, SRT Evans
Breast cancer research and treatment, 1994•Springer(OH) 2-Vitamin D 3, the active metabolite of vitamin D, is a secosteroid hormone with known
differentiating activity in leukemic cells. Studies have demonstrated the presence of vitamin
D receptors (VDR) in a wide range of tissues and cell types. Antiproliferative activity of 1, 25-
(OH) 2-vitamin D 3 has been documented in osteosarcoma, melanoma, colon carcinoma,
and breast carcinoma cells. This study was designed to analyze vitamin D receptor level in
breast cancer cells as a marker of differentiation and as a predictor of growth inhibition by 1 …
differentiating activity in leukemic cells. Studies have demonstrated the presence of vitamin
D receptors (VDR) in a wide range of tissues and cell types. Antiproliferative activity of 1, 25-
(OH) 2-vitamin D 3 has been documented in osteosarcoma, melanoma, colon carcinoma,
and breast carcinoma cells. This study was designed to analyze vitamin D receptor level in
breast cancer cells as a marker of differentiation and as a predictor of growth inhibition by 1 …
Summary
1,25-(OH)2-Vitamin D3, the active metabolite of vitamin D, is a secosteroid hormone with known differentiating activity in leukemic cells. Studies have demonstrated the presence of vitamin D receptors (VDR) in a wide range of tissues and cell types. Antiproliferative activity of 1,25-(OH)2-vitamin D3 has been documented in osteosarcoma, melanoma, colon carcinoma, and breast carcinoma cells. This study was designed to analyze vitamin D receptor level in breast cancer cells as a marker of differentiation and as a predictor of growth inhibition by 1,25-(OH)2-vitamin D3.
VDR messenger RNA was found to be present in relatively high levels in well-differentiated cells and in low levels in poorly differentiated cells. All cell lines had detectable VDR mRNA. Radiolabeled ligand binding assay showed a similar pattern. MCF-7 and T47D cells, which express VDR at moderate levels, showed significant growth inhibition by 10−9 M 1,25-(OH)2-vitamin D3 (p < 0.05). MDA-MB-231 cells, which have very low levels of VDR, demonstrated no growth inhibition by 1,25-(OH)2-vitamin D3 at concentrations up to 10−6 M. Based on these results it can be stated that VDR expression is lost with de-differentiation and that receptor is essential for the antiproliferative response to 1,25-(OH)2-vitamin D3.
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