Binding and degradation of low density lipoproteins by cultured human fibroblasts: comparison of cells from a normal subject and from a patient with homozygous …
JL Goldstein, MS Brown - Journal of Biological Chemistry, 1974 - ASBMB
125 I-Labeled low density lipoproteins were found to associate with monolayers of cultured
normal fibroblasts by two processes—one of high affinity and one of low affinity. The high
affinity association appeared to represent binding of the low density lipoprotein to specific
receptor sites on the cell surface. This binding process exhibited saturation kinetics at low
concentrations of the lipoprotein and competition by related molecules such as very low
density lipoproteins. In addition, this process was stimulated by the presence of calcium in …
normal fibroblasts by two processes—one of high affinity and one of low affinity. The high
affinity association appeared to represent binding of the low density lipoprotein to specific
receptor sites on the cell surface. This binding process exhibited saturation kinetics at low
concentrations of the lipoprotein and competition by related molecules such as very low
density lipoproteins. In addition, this process was stimulated by the presence of calcium in …