Nitric oxide oxidises a ferrous mammalian lipoxygenase to a pre-activated ferric species
R Wiesner, J Rathmann, HG Holzhütter, R Stöβer… - FEBS letters, 1996 - Elsevier
R Wiesner, J Rathmann, HG Holzhütter, R Stöβer, K Mäder, H Nolting, H Kühn
FEBS letters, 1996•ElsevierNitric oxide is known as an inhibitor of soybean lipoxygenase-1. Investigating the interaction
of a mammalian 15-lipoxygenase with nitric oxide, we found that this enzyme is also
inhibited reversibly when incubated with nitric oxide for a short time period (5 s) under
anaerobic conditions. This inhibition may be due to the formation of a dissociable
lipoxygenase-nitric oxide complex. With longer incubation periods the ferrous lipoxygenase
is oxidised to a ferric form. This oxidation renders the enzyme more susceptible to peroxide …
of a mammalian 15-lipoxygenase with nitric oxide, we found that this enzyme is also
inhibited reversibly when incubated with nitric oxide for a short time period (5 s) under
anaerobic conditions. This inhibition may be due to the formation of a dissociable
lipoxygenase-nitric oxide complex. With longer incubation periods the ferrous lipoxygenase
is oxidised to a ferric form. This oxidation renders the enzyme more susceptible to peroxide …
Nitric oxide is known as an inhibitor of soybean lipoxygenase-1. Investigating the interaction of a mammalian 15-lipoxygenase with nitric oxide, we found that this enzyme is also inhibited reversibly when incubated with nitric oxide for a short time period (5 s) under anaerobic conditions. This inhibition may be due to the formation of a dissociable lipoxygenase-nitric oxide complex. With longer incubation periods the ferrous lipoxygenase is oxidised to a ferric form. This oxidation renders the enzyme more susceptible to peroxide activation, causing a time-dependent shortening of the kinetic lag phase.
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