Biochemical and biophysical identification of cystic fibrosis transmembrane conductance regulator chloride channels as components of endocytic clathrin-coated …
NA Bradbury, JA Cohn, CJ Venglarik… - Journal of Biological …, 1994 - ASBMB
Cystic fibrosis results from mutations in the gene encoding the CFTR Cl-channel. Although
CFTR occurs as an integral component of the plasma membrane, recent studies implicate
CFTR in endocytic recycling and suggest that the protein may also exist in intracellular
vesicular compartments. To test this, we analyzed CFTR in clathrin-coated vesicles (CCV)
purified from cells constitutively expressing CFTR at high levels. CFTR immunoreactivity was
detected in CCV by immunoblot and was identified as CFTR based on labeling of …
CFTR occurs as an integral component of the plasma membrane, recent studies implicate
CFTR in endocytic recycling and suggest that the protein may also exist in intracellular
vesicular compartments. To test this, we analyzed CFTR in clathrin-coated vesicles (CCV)
purified from cells constitutively expressing CFTR at high levels. CFTR immunoreactivity was
detected in CCV by immunoblot and was identified as CFTR based on labeling of …