Immunoprecipitation and characterization of a binding protein specific for the peptide, intestinal trefoil factor
R Chinery, HM Cox - Peptides, 1995 - Elsevier
Recombinant rat intestinal trefoil factor (rITF) and human spasmolytic polypeptide (hSP)
were irreversibly cross-linked to specific binding sites in solubilized rat intestinal epithelial
membranes and human adenocarcinoma cells. Analysis of the immunoprecipitates by
immunoblotting identified a cross-linked protein complex of∼ 45 kDa, which under reducing
conditions appeared as a∼ 28-kDa band and the latter displayed ligand-stimulated
phosphorylation of a tyrosine, but not a threonine or serine, residue in the binding …
were irreversibly cross-linked to specific binding sites in solubilized rat intestinal epithelial
membranes and human adenocarcinoma cells. Analysis of the immunoprecipitates by
immunoblotting identified a cross-linked protein complex of∼ 45 kDa, which under reducing
conditions appeared as a∼ 28-kDa band and the latter displayed ligand-stimulated
phosphorylation of a tyrosine, but not a threonine or serine, residue in the binding …