[HTML][HTML] Hepatocyte growth factor/scatter factor binds with high affinity to dermatan sulfate
M Lyon, JA Deakin, H Rahmoune, DG Fernig… - Journal of Biological …, 1998 - ASBMB
We have demonstrated by affinity chromatography that hepatocyte growth factor/scatter
factor (HGF/SF) binds strongly to dermatan sulfate (DS), with a similar ionic strength
dependence to that previously seen with heparan sulfate (HS). Analysis of binding kinetics
on a biosensor yields an equilibrium dissociation constant, KD, of 19.7 nm. This corresponds
to a 10–100-fold weaker interaction than that with HS, primarily due to a faster dissociation
rate of the complex. The smallest DS oligosaccharide with significant affinity for HGF/SF by …
factor (HGF/SF) binds strongly to dermatan sulfate (DS), with a similar ionic strength
dependence to that previously seen with heparan sulfate (HS). Analysis of binding kinetics
on a biosensor yields an equilibrium dissociation constant, KD, of 19.7 nm. This corresponds
to a 10–100-fold weaker interaction than that with HS, primarily due to a faster dissociation
rate of the complex. The smallest DS oligosaccharide with significant affinity for HGF/SF by …